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It has been suggested that Protein Function in Cell Membranes be merged into this article or section. ()

Also see transmembrane protein.

A membrane protein is a protein molecule that is attached to, or associated with the membrane of a cell or an organelle. More than half of all proteins interact with membranes. Membrane proteins can be classified into two groups, based on the strength of their association with the membrane.

Main categories

Integral membrane proteins are permanently attached to the membrane. They can be defined as those proteins which require a detergent (such as SDS or Triton X-100) or some other apolar solvent to be displaced. They can be classified according to their relationship with the bilayer:

• Transmembrane proteins span the entire membrane. The transmembrane regions of the proteins are either beta-barrels or alpha-helical. The alpha-helical domains are present in all types of biological membranes including outer membranes. The beta-barrels were found only in outer membranes of Gram-negative bacteria, lipid-rich cell walls of a few Gram-positive bacteria, and outer membranes of mitochondria and chloroplasts.

• Integral monotopic proteins are permanently attached to the membrane from only one side.

Peripheral membrane proteins are temporarily attached either to the lipid bilayer or to integral proteins by a combination of hydrophobic, electrostatic, and other non-covalent interactions. Peripheral proteins dissociate following treatment with a polar reagent, such as a solution with an elevated pH or high salt concentrations.

Integral and peripheral proteins may be post-translationally modified, with added fatty acid or prenyl chains, or GPI (glycosylphosphatidylinositol), which may be anchored in the lipid bilayer.

Further information: Integral membrane proteins, Transmembrane proteins, Peripheral membrane proteins

Classification of membrane proteins to integral and peripheral does not include some polypeptide toxins, such as colicin A or alpha-hemolysin, and certain proteins involved in apoptosis. These proteins are water-soluble but can aggregate and associate irreversibly with the lipid bilayer and form alpha-helical or beta-barrel transmembrane channels. An alternative classification is to divide all membrane proteins to integral and amphitropic.¹ The amphitropic are proteins that can exist in two alternative states: a water-soluble and a lipid bilayer-bound, whereas integral proteins can be found only in the membrane-bound state. The amphitropic protein category includes water-soluble channel-forming polypeptide toxins, which associate irreversibly with membranes, but excludes peripheral proteins that interact with other membrane proteins rather than with lipid bilayer.

Further reading

• Protein-lipid interactions (Ed. L.K. Tamm) Wiley, 2005.
• Popot J-L. and Engelman D.M. 2000. Helical membrane protein folding, stability, and evolution. Annu. Rev. Biochem. 69: 881-922.
• Bowie J.U. 2005. Solving the membrane protein folding problem. Nature 438: 581-589.
• Cho, W. and Stahelin, R.V. 2005. Membrane-protein interactions in cell signaling and membrane trafficking. Annu. Rev. Biophys. Biomol. Struct. 34: 119–151.
• Goni F.M. 2002. Non-permanent proteins in membranes: when proteins come as visitors. Mol. Membr. Biol. 19: 237-245.
• Johnson J.E. and Cornell R.B. 1999. Amphitropic proteins: regulation by reversible membrane interactions. Mol. Membr. Biol. 16: 217-235.
• Seaton B.A. and Roberts M.F. Peripheral membrane proteins. pp. 355-403. In Biological Membranes (Eds. K. Mertz and B.Roux), Birkhauser Boston, 1996.
• Dürr U.H.N., Waskell L., and Ramamoorthy A. The cytochromes P450 and b5 and their reductases-Promising targets for structural studies by advanced solid-state NMR spectroscopy, 2007. BBA Biomembranes 1768: 3235-3259.

See also

• Integral membrane proteins
• Transmembrane proteins
• Peripheral membrane proteins
• Ion pump (biology)
• Carrier protein
• Ion channel
• Receptor (biochemistry) (including G protein-coupled receptor)
• Wikipedia:MeSH_D12.776#MeSH_D12.776.543_---_membrane_proteins

References

External links

• General Principles of Membrane Protein Folding and Stability from Stephen White laboratory
• Orientations of Proteins in Membranes (OPM) database 3D structures of integral and amphitropic membrane proteins
• MeSH Membrane+proteins

Wikipedia content modification information:

• This page was last modified on 11 November 2008, at 17:53.

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